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value, but if you assume the units are millimolar (mM), or micromolar ( M), the products/reactants ratio can be very different. A molar standard state is different from a millimolar standard state. You must remember the units that have been used (even though you ve just ignored them) and make sure that both Keq and the products/reactants ratio are expressed in the same units. Let s do a real example. The hydrolysis of ATP provides energy for virtually everything alive. The Keq for this reaction (ATP s ADP Pi) at 25 C and pH 7.0 is about 2.3 105 M. Notice that the concentration of water has also been ignored here. That s because it s large and relatively constant in biological systems, and putting it in would just change the numbers for everything by the same amount so it was decided to ignore it. With a Keq of 2.3 105 M, the G0 is 1.36 log10 (2.3 105) 7.3 kcal/mol. This would be the G if the products/reactants ratio were 1, but it s not. Let s assume that the local concentration of ATP in a cell is 5 mM, [ADP] is 60 M, and [Pi] is 5 mM (these are approximately right, but they will vary from cell to cell and at any given time in a cell). Keep in mind that this also means the amount of free energy available from ATP hydrolysis will vary from cell to cell and from time to time. With these concentrations, the products/reactants ratio becomes [ADP][Pi] [ATP] or products/reactants equation for G, G G With G0 G 6 (6 10 5 M) (5 10 (5 10 3 M) javascript pdf preview image Creating PDF thumbnails in JS with PDF JS - bl.ocks.org
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