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manipulated stressors involve both direct sympathetic and parasympathetic neural innervation, as well as indirect endocrine in uences via the circulation The list of elements of the immune system known to be in uenced by stress mechanisms, let alone the actual neural and endocrine pathways involved in these effects, seems to expand exponentially with each passing year (Ader, Felten, & Cohen, 2001; KiecoltGlaser et al, in press) In addition to underscoring the importance of current conceptual models of pathophysiology, this complexity poses basic problems in design and analysis In nearly all cases, state-of-the-art research by necessity assesses multiple correlated features of the stress response of interest This mosaic of interdependent outcomes often requires multiple statistical tests Atheoretical approaches to the control of Type I error rates (eg.

Franklin glances at his watch as he listens to the boarding announcement for his flight to Hong Kong He drops his empty coffee cup in a trash container, picks up his laptop, and strides through the corridors of Dulles Airport, heading toward Terminal C Though his cell phone is safely tucked in his jacket pocket, he scans the gate areas for a pay phone He has to make one more call before he leaves the country a very important call He finds a phone and dials the number The answering machine on the other end picks up, and he begins his well-planned message Sandy, I was hoping to catch you to ask a quick question I wondered how you like your IBM ThinkPad A22p laptop Anyway, I hope all is well I ll talk to you when I get back He hangs up and heads to Gate C-23.

, Bonferroni corrections) are one option for dealing with multiple dependent variables, but this method results in a potentially severe loss of statistical power Given the time and expense involved in many studies of this type, overly conservative approaches to the problem are ill-advised Clear, conceptually driven, a priori organization or prioritization of the list of outcomes can reduce the problem to some extent Further, the use of multivariate procedures and the interpretation of composite outcomes can be useful (Huberty & Morris, 1989) The least acceptable approach, however, is the assessment of multiple components of a complex system without a priori rank ordering of their importance, followed by consideration of the unadjusted, signi cant effects among many signi cance tests computed Psychobiological mechanisms are generally conceptualized in two very distinct ways in this area of research.

Biofeedback Techniques EMG Biofeedback Kotses and his colleagues (Glaus & Kotses, 1983; Kotses et al., 1991) hypothesized that changes in facial muscle tension directly produce respiratory impedance through a trigeminalvagal re ex pathway (such that tensing these muscles produces bronchoconstriction, while relaxing them produces bronchodilation). They tested the model using frontal EMG biofeedback to increase and decrease tension in the facial muscles. Frontal EMG relaxation training was found to decrease facial muscle tension and to produce improvements in pulmonary function, while training to increase tension in this area had the opposite effect. EMG biofeedback training to the forearm muscles had no effects. Several studies from other laboratories have failed to replicate these ndings, however (Lehrer et al., 1994, 1996; Lehrer, Generelli, & Hochron, 1997; Mass, Wais, Ramm, & Richter, 1992; Ritz, Dahme, & Wagner, 1998). Another biofeedback strategy, suggested by Peper and his colleagues, linked pulmonary function with tension in the skeletal muscles of the neck and thorax (Peper & Tibbetts, 1992). (Tension in this area often is produced by a pattern of thoracic breathing.) They used EMG biofeedback training to teach participants to relax these muscles, while simultaneously increasing volume and smoothness of breathing. This training was done in the context of a multi-component treatment that included desensitization to asthma sensations and training in slow diaphragmatic breathing. The latter training was carried out by a biofeedback procedure using an incentive inspirometer. At the follow-up, all subjects signi cantly reduced their EMG tension levels while simultaneously increasing their inhalation volumes. Subjects reported reductions in their asthma symptoms, medication use, emergency room visits, and breathless episodes. A small study from our laboratory did not show signi cant effects for this method